Ecological problems such as environmental degradation are urgent problems to be solved in karst areas. It is of great theoretical and practical significance to explore the landscape pattern and the relationship between landscape pattern and ecosystem service value (ESV) in karst areas. Based on the remote sensing data of Guiyang City, China, this paper uses landscape index method to evaluate landscape pattern, uses value equivalent factor method to estimate ESV, and uses gray correlation analysis to analyze the relationship between landscape pattern and ESV from patch type level and landscape level. The results showed that: (1) the landscape fragmentation slowed down, the complexity decreased, and the stability increased. (2) The total value of ESV shows an upward-downward-upward trend. Spatially, it showed a law of geographical center as the core, low in the middle and high in the surroundings. (3) ESV value is closely related to landscape aggregation and shape complexity.