The forests in China's temperate semi-arid region play a significant role in water conservation, carbon storage, and biodiversity protection. An accurate estimation of their aboveground biomass (AGB) is crucial for assessing key ecological characteristics, such as forest carbon storage capacity, biodiversity, and ecological productivity. This provides a scientific basis for forest resource management and ecological conservation in this region. In this study, we extract 17 features related to the dominant species (Larix gmelinii and Betula platyphylla), including 7 vegetation indices derived from remote sensing data, 14 indices from 7 satellite bands, and 3 forest site characteristics. We then analyze the correlations between the AGB and these features. We compare the performance of AGB estimation models using linear regression (LR), polynomial regression (PR), ridge regression (RR), Support Vector Regression (SVR), Extreme Gradient Boosting (XGBoost), and random forest regression (RFR). The results show that for Larix gmelinii, the Landsat 8 bands TM4 and TM7 have a greater degree of correlation with the AGB than the other features, while for Betula platyphylla, bands TM3 and TM4 show a greater degree of correlation with the AGB, and elevation has a weaker correlation with the AGB. Although the linear regression (LR) demonstrates certain advantages for AGB estimation, particularly when the AGB values range from 40 to 70 t/ha, the RFR outperforms in overall performance, with estimation accuracies reaching 85% for Betula platyphylla and 89% for Larix gmelinii. This study reveals that both the species and environmental characteristics may significantly influence the selection of the remote sensing features for AGB estimation, and the choice of algorithm for model optimization is critical. This study innovatively extracts the features related to the dominant species in temperate forests, analyses their relationships with environmental factors, and optimizes the AGB estimation model using advanced regression techniques, offering a method that can be applied to other forest regions as well.