Water scarcity is becoming an alarming issue in the Mediterranean countries. Therefore, using the treated wastewater in the irrigation is considered as a valuable option. However, uncontrolled and long-term irrigation by wastewater leads to human health and environmental damages, mainly related to some specific pollutants. The assessment of the availability and toxicity of the heavy metals after long term irrigation, under semi-arid climate, is not yet well documented. In this study, physicochemical properties, genotoxicity (Vicia faba micronucleus test), total and available (CaCl2-extractable) concentrations of Cr, Pb, Cu, Zn, Co and Cd in eight soils of peri-urban farms irrigated with wastewater were examined to evaluate their accumulation. The results indicated that long-term irrigation with wastewater induced significant increase of electrical conductivity, organic matter, calcium carbonate equivalent and nutrient availability. Total and available concentration of heavy metals were significantly higher (P < 0.05) in irrigated soils by wastewater. The total concentrations of Zn, Pb, Cu, Cr, Cd and Co in irrigated soils by wastewater at 0-40 cm depth were 85.69, 43.94, 34.86, 14.62, 9.94 and 7.17 mg kg(-1), respectively. Furthermore, the increase of the available metal fraction in irrigated soils by wastewater at 0-40 cm depth followed the following order: Co (1270.1%) > Cd (914.5%) > Cu (881.5%) > Cr (471.2%) > Pb (230.8%) > Zn (223.8%). The micronucleus assay indicated significant increase of micronucleus frequencies (41.25 parts per thousand, 35.48 parts per thousand, 21.66 parts per thousand, 16.23 parts per thousand and 13.62 parts per thousand respectively for P1, P2, P3, P4 and P7) which were higher than the negative control (0 parts per thousand) and the irrigated soil by fresh water (3.29 parts per thousand). The micronucleus induction was significantly correlated with the high available fraction of Cd, Co and Zn at P1, P2 and P7. The genotoxicity can be a powerful test to assess the ecological effects associated with the interactions of heavy metals with other pollutants. (C) 2021 Elsevier B.V. All rights reserved.