Soil moisture and nitrogen (N) fertilizer control the balance between nitrous oxide (N2O) production and consumption in soil. However, the impact of nitrification and urease inhibitors on soil N2O production and consumption under varying soil moisture levels remains insufficiently understood. In this study, a soil column experiment was conducted to investigate N2O concentrations and accumulation at different soil depths (0, 5, 15, 30, and 60 cm) in sandy soil over two months. The soil was collected from a drip-irrigated field subjected to longterm cotton cultivation in an arid region. Four fertilizer treatments were applied: urea, urea + nitrification inhibitor (Dicyandiamide, DCD), urea + nitrification and urease inhibitors [N-(n-butyl) thiophosphoric triamide, NBPT], and an unfertilized control. These treatments were tested under three soil moisture levels [23%, 46%, and 70% water-filled pore space (WFPS)]. The results showed that treatments with inhibitors significantly reduced cumulative surface N2O emissions by 33.2%-58.2% compared to urea alone. Notably, the treatment with both DCD and NBPT achieved the greatest reductions in surface emissions and in N2O concentrations at 5 cm and 15 cm soil depths at 70% WFPS. Additionally, across all N fertilizer sources, 70% WFPS led to increased N2O concentrations in the soil profile compared to lower soil moisture levels. Column N2O accumulation was positively correlated with surface cumulative N2O emissions under all three moisture conditions. Our study highlights that under high soil moisture conditions, DCD and NBPT can significantly mitigate intense N2O emissions in sandy soils. This emission reduction is likely attributed to enhanced nitrification within the 0-15 cm soil layer rather than reduced N2O diffusivity and denitrification along the soil profile. Therefore, we recommend applying nitrification and urease inhibitors under high soil moisture conditions as an effective strategy to reduce N2O emissions in sandy soils.