The objective of this study was to evaluate the desertification vulnerability of an approximately 1,000-ha area in Anatolia, Turkey, comprising semiarid oak (Quercus spp.) and pine (Pinus spp.) forests and the adjacent cultivated lands by using the environmental sensitivity index (ESI). We calculated the ESI values for 632 randomly selected sites in the study area by entering the desertification indicators vegetation cover, plant type, soil depth, rock fragments, soil texture, slope gradient, drainage, parent material, mean annual precipitation, slope aspect, aridity index, land use intensity, and policy enforcement into the web-based model Desertification Indicator System for Mediterranean Europe. The spatial structure of the ESI values was then evaluated using semivariograms and kriging-interpolated surface maps. The mean ESI value was significantly greater in the cultivated areas (1.47; range=1.25-1.61) than in the pine (1.32; 1.03-1.45) and oak (1.28; 1.18-1.57) forests (P<0.01). The ESI was moderately spatially dependent in the oak forests and cultivated areas and strongly spatially dependent in the pine forests, as evidenced by the corresponding semivariograms. The ordinary kriging-interpolated maps showed that most of the cultivated areas were critically vulnerable to desertification, whereas the majority of the forested areas were moderately vulnerable. We examined how the ESI values varied with soil organic matter (OM) content as an independent variable and found that the OM content was significantly moderately correlated with the ESI (n=632, r=-0.51, P<0.01), suggesting that the ESI could be used to evaluate the desertification vulnerability of similar landscapes across Turkey.