2018-08-01 TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI 2018 43(卷), 4(期), (417-424页)
Aim: In this research, the effects of two elicitors [jasmonic acid (JA) and salicylic acid (SA)] on the accumulation of essential oils in the seed cultures of Sage (Salvia officinalis L) were studied. Materials and methods: This research was conducted at the research field of Islamic Azad University, Shahrekord branch located at 50 degrees 56/E longitude, 32 degrees 18/N latitude during 2015 and 2016. The study area was classified as semi cold with an average temperature of 11.5 degrees C and semi-arid with 329.9 mm of average rainfall. Seeds of sage were planted under field conditions. The following treatments were applied after the plants had four leaves: water, acetone, JA (0.1051, 0.2102, 0.4204, 0.8408 g/L), SA (0.0138, 0.138, 0.276, 0.552 g/L) and control. Results: Twenty-seven essential oils were obtained and the most notable were: alpha-pinene, limonene, beta-pinene, camphor, thymol, camphene, thujone-trans, thujonecis, 1,8-cineole, borneol, borneol acetate, carvacrol, alpha-humulene, caryophyllene. JA was more effective in stimulating the accumulation of a-pinene, limonene, beta-pinene, camphor, thymol, camphene, thujone-trans, thujone-cis, 1,8-cineole, borneol, borneol acetate, carvacrol, alpha-humulene and caryophyllene. Conclusion: JA and SA had increasing effects on essential oils. The best treatments were found to be JA 0.1051 g/L, JA 0.2102 g/L and SA 0.138 g/L.