To contribute to national greenhouse gas emissions (GHG) reduction targets, grain growers need strategies that minimise emissions associated with grain production. We used life cycle assessments (LCAs) with field-measured production inputs, grain yields and proteins, legume nitrogen (N-2) fixation, and soil nitrous oxide (N2O) and methane (CH4) emissions, to explore mitigation strategies in 3-year crop sequences in subtropical Australia. The sequences were: canola plus 80 kg/ha fertiliser nitrogen (80N)-wheat 85N -barley 65N (Ca(N)Wt(N)Ba(N)), chickpea 0N-wheat 85N-barley 5N (CpWt(N)Ba), chickpea 0N-wheat 5N-chickpea 5N (CpWtCp), and chickpea 0N-sorghum 45N (CpSg(N)). We also assessed the impacts of split fertiliser N application and urea coated with DMPP, a nitrification inhibitor, on the LCA for the Ca(N)Wt(N)Ba(N) sequence. Total pre-farm plus on-farm GHG emissions varied between 915 CO2-e/ha (CpSg(N)) and 1890 CO2-e/ha (Ca(N)Wt(N)Ba(N)). Cumulative N2O emitted over the 3-year study varied between 0.479 kg N-2 O-N/ha (CpWtCp) and 1.400 kg N2O-N/ha (Ca(N)Wt(N)Ba(N)), which constituted 24-44% of total GHG emissions. Fertiliser production accounted for 20% (CpSg(N)) to 30% (Ca(N)Wt(N)Ba(N)) of total emissions. An extra 4.7 kg CO2-e/ha was emitted for each additional kg N/ha of applied N fertiliser. Three-year CH4 emissions ranged from -1.04 to -0.98 kg CH4-C/ha. Split N and DMPP strategies could reduce total GHG emissions of Ca(N)Wt(N)Ba(N) by 17 and 28% respectively. Results of the study indicate considerable scope for reducing the carbon footprint of subtropical, dryland grains cropping in Australia.