Zinc (Zn) and boron (B) deficiency is one of the most widespread micronutritional disorders in crops, and occurs predominantly in calcareous soils of arid and semiarid regions. A field experiments with maize plant grown on Zn and B deficient soil was carried out in a calcareous soil at Fars Province, Iran. This work aimed to study the interaction effect of Zn and B on the concentration and uptake of Zn and B in the leaf during 2010. Treatments including five levels of Zn (0, 8, 16 and 24 kg ha(-1) and Zn foliar spray) and four levels of B (0, 3, and 6 kg ha(-1) and B foliar spray) in a completely randomized block design were set up. The findings showed that application of Zn, B, and Zn-B interaction on the B concentration in the leaf was insignificant. Zinc spraying increased Zn concentration in the leaf but adding Zn to the soil had no significant effect on it. Presence of B prevented from the increase in Zn concentration in the leaf; that is, an antagonism was seen between the Zn and B. Boron application decreased leaf Zn content and this increase was not influenced by the amount of Zn. Zinc use at no presence B in the soil (zero B and B spraying levels) increased leaf Zn content. [F. Aref. Concentration and uptake of zinc and boron in corn leaf as affected by zinc sulfate and boric acid fertilizers in a deficient soil. Life Science Journal 2011;8(1):26-32]. (ISSN: 1097-8135). http://www.lifesciencesite.com.