To study the effect of silage feeding on growth performance, microbial nitrogen synthesis, meat quality and fatty acid profile of growing lambs, an experiment conducted on fifty-four lambs of Avishaan strain under randomized block design. The lambs were randomly divided into three group of eighteen lambs (n = 18) in each, experimental period was 90 days (3-6 month of age) and the initial average weight of the lamb was 16.8 +/- 0.09 kg. All the lambs were offered 300 g concentrate daily and ad libitum roughage. Roughage in control (C) group was dry chaffed Cenchrus ciliaris hay, in test group T-I dry chaffed cenchrus hay plus silage on equal dry matter basis whereas in test group T-II only fresh silage. During the experimental period nutrient utilization and rumen fermentation studies were undertaken. After six months of age, eight representative male lambs from each group were slaughtered to study the carcass traits, quality and muscle fatty acid composition. Results revealed higher(P < 0.05) digestibility of dry matter, organic matter and fibre fractions in T-I and T-II group compared to C lambs. The microbial nitrogen synthesis per kg of digestible organic matter intake was higher (P < 0.05) in T-II group. Rumen fermentation revealed higher (P < 0.05) total nitrogen and TCA-ppt-N in T-II followed by T-I and C groups. However, TVFA was lower in T-II and T-I compared to C group. Carcass data revealed lower (P < 0.05) quantity of ingesta as well as lower (P < 0.05) carcass fat with silage feeding in T-II and T-I compared to C group lambs. Values of TBARS in meat were significantly lower (P < 0.05) in T-II and T-I group compared to C group, and antioxidant activity showed a corresponding increase with silage feeding, being highest in the T-II group, followed by T-I, and lowest in the C group. Longissimus thoracis muscle fat revealed higher (P < 0.05) proportion of C16, C18 and lowered (P < 0.05) that of C18:1n9c fatty acids with silage feeding, consequently the proportion of SFA was increased and that of MUFA decreased with silage feeding. The proportion of n-3 fatty acids was increased with silage feeding in T-II and T-I group. The ratio of n - 6/ n-3 and C18 desaturase activity was lower (P < 0.05) whereas C16 to C14 thioesterase activity was higher(P < 0.05) with silage feeding in T-II and T-I compared to C group lambs. Based on the findings of this experiment, it can be concluded that in arid and semi-arid regions, silage feeding serves as an eco-friendly strategy for small ruminant production and supports better shelf life of meat, reduced fat in the Longissimus thoracis muscle, with increased proportion of n-3 fatty acids. However, for achieving better weight gain and improved feed conversion efficiency, a combination of grass hay and silage on an equal dry matter basis is recommended.