Terminal temperature stress is considered one of the major challenges that occur during the flowering period and leads to a reduction in crop yield. Climate change poses a significant obstacle. This study was conducted at the Students' Instructional Farm (SIF) ofANDUAT, Ayodhya, while laboratory analyses took place in the Crop Physiology Laboratory during the rabi season of 2023-24. Ayodhya district has a semi-arid climate with an average annual rainfall of 1100 mm, of which around 80 % occurs during the monsoon season (June to September). Three wheat varieties viz., PBW-343, HD-2967 and Kundan were evaluated in 3 replications and 3 different dates of sowing using split plot design (SPD). In contrast, Kundan is a late-sown, high-yielding variety known for its tolerance to high-temperature stress. The value for the highest plant height was recorded 83.56 cm and at late sowing (30th December). When the sowing was done on 30th November then at 60 days the total chlorophyll contents was 2.19, 2.2 and 1.9 (mg g-1 FW), at 15th December 53.89, 54.75 and 51.2 (mg g-1 FW) and at 30th December 1.92, 2.0, 2.11 (mg g-1 FW) in all 3 varieties of wheat. The results revealed on elucidating the effects of terminal temperature stress on the physio-biochemical traits of wheat (Triticum aestivum L.). Also considering the physiochemical parameters will also affect the yield of wheat significantly.