Sorghum (Sorghum bicolor) is considered as a high-yielding, low-water requiring fodder crop suitable for feeding livestock reared in semi-arid climates of Asian countries. However, its energetic potential for ruminants could not be exploited owing to high fibre and lignin contents, resulting in inefficient utilization by rumen microbes. A low lignin brown mid rib sorghum (SPV- 2018/ CSV 43 BMR/ Jaicar Nutrigraze) cultivar developed by the pedigree method of breeding was evaluated for its nutrition quality, carbohydrate fermentation kinetics and methane production by in vitro rumen fermentation technique and assessed the feeding value in lactating Murrah buffaloes (Bubalus bubalis) in comparison to non-bmr sorghum, MFSH-4. The in vitro gas production and kinetics experimentations of both cultivars were conducted with buffalo rumen fluid in graduated glass syringes. The feeding value of these cultivars was evaluated in twenty lactating Murrah (Bubalus bubalis) buffaloes (body weight, 568.10 +/- 55.09 kg; milk yield 12.96 +/- 1.17 kg) allocated into two groups (n = 10) in a completely randomized design during a feeding trial of 105 days. Along with the concentrate mixture, the treatment group animals (SPV2018) received the entire roughage source from bmr sorghum fodder, whereas control group animals (MFSH-4) were fed a conventional cultivar. The chemical composition of bmr sorghum cultivar, SPV-2018, demonstrated lowered (p < 0.01) lignin (ADL) and fibre (NDF and ADF) contents, in comparison to MFSH-4. An enhanced (p < 0.05) rate of fermentation (c), feed degradability and volatile fatty acids production were recorded along with higher production of carbohydrate-degrading enzymes (xylanase, beta-glucosidase, acetyl esterase) and fibrolytic bacterial colonization on SPV-2018 fermentation. Compared to MFSH-4 cultivar, SPV-2018 exhibited a significant increase (p < 0.05) in feed digestibility and methane production (ml/g DDM). There was improved (p < 0.05) digestibility and nutritional quality of the total ration in buffaloes fed SPV-2018 sorghum. The greater (p < 0.05) daily milk production as well as fat corrected milk (6% FCM) in buffaloes manifested an enhanced feeding value of novel bmr sorghum cultivar (SPV-2018) over conventional sorghum, MFSH-4. The study signified the advantages of bmr sorghum (SPV-2018) for its larger applications among the farmers of the region for enhancing livestock productivity.