Biodegradable plastics, proposed as sustainable alternatives of conventional plastics, pose risk to soil ecosystems and play critical roles in regulating soil greenhouse gas emission. Yet, how biodegradable microplastics (MPs) exposure affect the soil nitrous oxide (N2O) emission and underlying mechanisms remains poorly unclear. This study investigated the effects of biodegradable (polylactic acid, PLA) MPs and conventional (polyethylene, PE) MPs on soil N2O concentration and emissions using a soil column experiment. Soil N2O concentration and emission were measured during N2O "hot moment" events induced by simulated rainfall events, alongside high-frequently monitor of soil oxygen (O2) concentration. Simultaneously, microbial functional genes related to nitrification and denitrification were measured to compare their contribution to N2O production. We found that exposure of MPs triggered N2O pulse by reducing O2 concentration, and cumulative N2O emissions in PLA treatments increased by 2.3-2.8 folds higher than their counterparts in PE treatments. Consumptions of O2 caused by MPs exposure was dose-dependent, with minimum O2 concentration ranged 16.2-18.3 % for PE treatments and 4.0-12.0 % for PLA treatments, respectively. Denitrification emerged as the primary contributor of increased N2O concentration, as evidenced by increased in nirK and nirS genes, particularly when soil O2 concentrations dropped to 7.2-12.3 % in PLA treatments. Overall, our findings underscore biodegradable MPs could intensity O2 consumption and N2O production, offering critical insights for risk management strategies and guiding the application of biodegradable plastics in future agricultural contexts.