It was crucial to quantify the soil infiltrability after land use conversion and vegetation change. However, the impact mechanism and differences of revegetation on soil infiltrability in subsoil and topsoil remain unclear. The soil infiltrability in topsoil (0-20 cm), upper subsoil (20-40 cm) and deep subsoil (40-60 cm) was analyzed using permeameters with a fixed head height in four typical land use types on the tableland and gully-slope in the Wangdonggou watershed of the Loess Plateau in China. There were obvious differences in soil saturated hydraulic conductivity (Ks) among different plant communities, and in the Ks between tableland and gully-slope (P < 0.01). Our study quantified the differences in soil infiltrability among soils at different depths, and explored the response of soil infiltrability at different depths to revegetation. Revegetation enhanced soil infiltrability through regulating soil properties and the combination of root systems. The basic soil property and vegetation characteristic accounted for 53.1% and 16.6% of the variation of Ks, respectively. The interaction between vegetation and soil properties also accounted for 14.7%. Topsoil infiltrability exhibited greater sensitivity to revegetation than subsoil. Compared with bare land, revegetation on the gully-slope increased the infiltrability of topsoil by 4.22 times, but the infiltrability of deep subsoil only increased by 2.28 times. The soil infiltrability for all land-use types reduced with the increase of soil depth. Compared with the topsoil, the average Ks of deep subsoil on the tableland and the gully-slope decreased by 62.3% and 78.1%, respectively. Our work can offer a novel perspective for understanding the soil hydrological processes.