Water erosion is a major driver of soil degradation in arid and semi-arid regions, where the lack of vegetative cover and intense rainfall accelerate erosion processes. Field experiments were conducted to evaluate the effectiveness of stone walls (SW) as a soil conservation practice in reducing soil erosion using the universal soil loss equation. Furthermore, the support practice factor (P) was estimated via integrating computational measurements of changes in A horizon thickness with slope profiles. Six sites with varying slope gradients (8%, 10%, 15%, and 25%) implementing SW were compared to neighboring sites lacking this practice in the northeastern parts of Jordan. SW reduced average annual soil loss by 83%, lowering the average annual erosion rate from 58 t.ha-1.yr-1 (severe risk) to 10 t.ha-1.yr-1 (slight risk). The implementation of SW stabilized the thickness of the A horizon and organic matter contents across different slope gradients. In contrast, the absence of SW led to greater soil displacement and accumulation of organic matter at the lower slopes, indicating higher erosion risks. The average estimated P factor was 0.35. These findings underscore the effectiveness of conservation practices in controlling soil erosion, enhancing soil quality, and promoting sustainable land use in arid and semi-arid environments. Wider adoption of such measures can significantly contribute to combating soil degradation and improving agricultural productivity in similar regions worldwide.