Effective water governance is essential for sustainable development amidst water scarcity challenges in semi-arid regions like Esmeraldas Province, which has substantial agrotourism potential. Yet, fragmented governance and chronic water shortages threaten its viability. Using a mixed-method approach, this study analyzed how sustainable water governance can support agrotourism development in Esmeraldas Province, Ecuador. This study combined policy gaps analysis, stakeholder surveys (policymakers, farmers, community leaders, and tourism operators), and water availability using the Standardized Precipitation Evapotranspiration Index (SPEI) from 1980 to 2022. The results revealed a lack of policy regulation and water infrastructure as the major governance gaps that need more intervention. The survey respondents indicated that water is mainly used for domestic and economic activities and the conservation of natural ecosystems. The SPEI revealed a significant drought trend falling below -3, with severe drought years coinciding with many crop losses and a fall in tourism. This study highlights the interconnection between water governance and agrotourism in Esmeraldas, Ecuador, proposing a strategic framework that incorporates adaptive governance principles and inclusive participation mechanisms, emphasizing targeted capacity building to strengthen water management practices and enhance the Sustainable Development Goals for agrotourism resilience.