Conservation tillage is widely recognized as a key practice for improving soil quality in agriculture. However, despite growing interest, the combined effects of conservation tillage and intercropping on soil properties remain underexplored, particularly in semi-arid regions. There are three tillage practices: conventional tillage (CT), minimum tillage (MT), and reduced tillage (RT), and two strip intercropping systems, wheat-chickpea (W-C) and wheat-brassica (W-B), and three sole crop systems, sole-wheat (SW), sole-chickpea (SC), and sole-brassica (SB). In the present study, we investigated the effects of various tillage systems and strip intercropping on soil properties in a newly implemented site where conservation tillage practices were adopted. The results showed that MBC contents were affected by tillage, cropping systems, and soil depth. A significant increase in MBC concentrations occurred in wheat-chickpea and sole-chickpea (197 and 196 mu g g- 1, respectively) under the RT system at 0-15 cm soil depth. Bulk density (BD) and nitrate-nitrogen (NO3-N) gradually increased with the tillage treatments from CT to RT. According to the model's findings, implementation of reduced tillage (RT) will result in elevated levels of microbial biomass carbon (MBC) by the year 2100. Ultimately, implementing conservation tillage practices and strip intercropping can contribute to sustainable agricultural systems by protecting soil resources, enhancing soil microbial activity, and supporting long-term productivity.