Evapotranspiration (ET) variation is typically influenced by climatic factors, which are considered the primary drivers of agricultural water requirements. Any changes in ET rates directly affect crop water demands. In this study, temporal trends and magnitudes of key climatic variables, and their impacts on reference evapotranspiration (ETo) during 1981-2020, were evaluated across 36 districts of Punjab, Pakistan. Positive serial correlations, ranging from 0.29 to 0.48, were identified and removed using the pre-whitening technique. Increasing trends in maximum temperature (Tmax) and wind speed (WS) across Punjab and its subregions were observed, while relative humidity (RH) exhibited both increasing and decreasing trends. No significant trends were detected for the minimum temperature (Tmin). On a monthly scale, in the Southern Punjab (SP) region, Sen's slope estimated an increase in ETo, ranging from 0.239 mm/year in November to 0.636 mm/year in May, at a significance level of alpha = 0.05 (5%). At the provincial scale, significant upward trends in ETo were observed for the annual, Kharif, and autumn seasons, with Z-values of 2.04, 2.16, and 3.13, respectively, at alpha = 0.05 and 0.01. It was determined that, on an annual scale in Punjab, ETo sensitivity to climatic parameters followed the following order: Tmax > wind speed (WS) > Tmin > RH. The best-fitted models for Tmax, Tmin, WS, and RH were Gaussian, exponential, and spherical. ETo was found to increase spatially from North to South Punjab, with an approximate rise of 70-80 mm/decade. The results provide a scientific basis for understanding hydroclimatic drivers of ETo in semi-arid regions and contribute to improving climate impact assessments on agricultural water use. The observed ETo increases, particularly in South Punjab and lower Central Punjab, highlight the need for region-specific irrigation scheduling and water allocation. These findings can guide cropping calendars, improve irrigation efficiency, and increase canal water supplies to high-ETo areas, supporting adaptive strategies against climate variability in Punjab.