Desert plants develop unique functional traits and resource utilization strategies under environmental stress, among which, water and nitrogen utilization strategies are the key resource utilization strategies for desert plants. Research on plant water and nitrogen utilization and leaf functional traits has rarely involved high-altitude desert shrubs. The synergistic or trade-off relationship between water and nitrogen utilization in desert shrubs remains unclear, and the variation patterns of leaf functional trait combinations related to water and nitrogen utilization along environmental gradients urgently need to be studied. This study takes the typical desert shrubs in the eastern part of the Qaidam Basin on the Qinghai-Tibet Plateau in China as the research object, selects the stable carbon and nitrogen isotopes (delta 13C, delta 15N) of plant leaves to characterize the water use efficiency (WUE) and nitrogen use strategy (NUE) of plants, explores the main leaf functional traits related to water and nitrogen utilization, and analyzes the relationship between leaf functional traits and environmental factors. The results show that the resource utilization traits of desert shrubs can be divided into two groups: water and carbon utilization centered on delta 13C and nutrient utilization centered on delta 15N. There are synergistic or trade-off relationships among plant functional traits. There is a trade-off relationship between water and nitrogen utilization in plants. The leaf functional traits related to water and nitrogen utilization in plants form a "water and nitrogen utilization leaf economic spectrum" along the gradients of temperature, drought, salinity, and nutrients. In conclusion, desert plants adapt to the environment of high cold, drought, high salt content, and limited nutrients by adjusting the relevant leaf functional traits. This study combines the stable carbon and nitrogen isotopes of plant leaves with the combined characteristics of leaf functional traits under different environmental gradients, providing a new perspective for understanding the water and nitrogen utilization strategies of high-altitude desert shrubs and their adaptation mechanisms to arid environments.