Habitat fragmentation represents a significant threat to biodiversity, particularly the survival of wild species. Constructing and optimizing ecological networks are critical for promoting sustainable biodiversity, especially in the conservation of unmanaged wildlife. To address this, this study focused on designing and optimizing an ecological network tailored to the preservation of the Xinjiang desert lacertid lizard (Eremias multiocellata). This study integrated a dual-model approach, applying the InVEST model for habitat quality assessment and the MaxEnt model for suitable habitat prediction. An overlay analysis identified 15 core ecological source areas spanning 126,044 km2, primarily located in the desert-grassland transition zones of the central and western study areas. A total of 34 ecological corridors were established utilizing the minimum cumulative resistance model, totaling 3764 km in length. These include 11 long corridors, 17 short corridors, and 6 potential corridors. Additionally, 100 strategic points were identified: 41 pinch points, 38 barrier points, and 21 stepping stones. This study identifies priority areas and obstacles affecting the ecological connectivity of the species' habitats and highlights the importance of small habitat patches for long-term species dispersal and habitat expansion, providing more comprehensive guidance for sustainable development and species conservation. Furthermore, the methodology provides valuable insights into biodiversity conservation and the optimization of the natural habitat spatial layout in desert ecosystems, along with novel methods for managing and conserving other unmonitored animal species in various ecosystems.