Over thousands of years, most agricultural fields have experienced significant degradation. Regenerative agriculture offers an effective approach to restoring soil and plant quality while enhancing productivity through nature-based solutions. This study investigated the effects of different doses of commercial cocktail mycorrhizal fertilizer on plant growth, nutrient content, and biomass yield of stevia under semi-arid conditions. Enhancing symbiotic cycles between microorganisms and plants is a key regenerative agriculture method. One of the most important is the symbiosis between plant roots and soil fungi, known as mycorrhiza. Mycorrhiza applications are increasingly common in regenerative agriculture. Two doses of mycorrhizal fertilizer (2.5 g plant(-1) and 5 g plant(-1)) were applied, with a control group receiving no mycorrhiza. Plant height, fresh weight, total fresh yield, leaf dry weight, branch dry weight, total dry yield, SPAD readings, nutrient contents, total steviol glycoside, and rebaudioside A were measured. Results showed that ERS treatments significantly (p < 0.01) increased plant height compared to the control. The ERS2 group had the highest branch numbers, with averages of 12.2 in the first year and 17.0 in the second year. The fertilizer's impact on growth was more pronounced in the second year, showing cumulative benefits. Increased branch numbers and plant height led to higher fresh and dry biomass yields. Mycorrhizal fertilizer enhanced nutrient content in the leaves, suggesting its suitability for stevia in semi-arid conditions, offering alternative income potential for farmers.