Understanding soil respiration (Rs) dynamics in Mediterranean olive groves is crucial for quantifying carbon fluxes under climate change. Soil respiration represents the combined CO2 efflux from root metabolic activity and microbial decomposition of soil organic matter, processes strongly controlled by soil moisture, temperature, and the quantity and quality of organic matter inputs in semi-arid Mediterranean environments. This study quantified the seasonal and spatial variability of Rs in a traditional rainfed olive orchard planted at a spacing of 11 m between rows and 9 m between trees (approximate to 101 trees ha-1). Continuous measurements were conducted in two contrasting zones, under-canopy (UC) and inter-row (IR), using automated soil CO2 flux chambers. Annual Rs reached 3.68 Mg CO2 ha-1 y-1 in UC and 2.21 Mg CO2 ha-1 y-1 in IR, with substantially higher emissions per unit area beneath the canopy. However, due to its larger surface proportion, the IR zone contributed more to the orchard scale CO2 budget. Soil water content emerged as the dominant environmental driver of Rs, moderating or suppressing the temperature response during dry periods. These findings highlight the importance of explicitly considering microsite heterogeneity when assessing soil CO2 efflux and designing sustainable carbon-management strategies in Mediterranean olive agroecosystems.