The aim of this study was to assess the long-term adaptation and growth performance of 50 species introduced in 1991 on the island of Porto Santo, Madeira Archipelago, in order to guide afforestation and soil restoration under the island's arid conditions, especially in biosphere reserves. The experiment was conducted in Alentejo, Pico Juliana and Matinho, three sites with different types of elevation, soil and exposure. A total of 502 experimental units (five plants each) were established with a completely randomized design in the three sites in 1991 to test the adaptation of 50 species from Mediterranean, African, Australian and American dry climates. Plants were grown in local nursery conditions and planted in rows with 1 x 4 m spacing. Soil properties were analyzed, and survival and growth (height and stem diameter) were monitored in 1991, 1992 and 2025. An analysis of variance was performed for the whole experiment, with the three sites showing significant differences in survival and height among species and sites thirty-four years after the planting. Some species showed high survival and growth, such as Pinus halepensis, Eucalyptus sideroxylon and Casuarina cunninghamiana. Others, like Schinus terebinthifolius and Thevetia neriifolia, showed good adaptation, and invasive behavior at the best sites, but their performance was strongly dependent on site conditions, with Alentejo being the most limiting site. This study demonstrates the long-term value of forest experiments and of long-term monitoring, providing rare data on species adaptation under semi-arid insular conditions. The findings support future afforestation strategies focusing on ecological suitability and invasiveness risk.