Kohat, located in northern Pakistan, holds ecological importance due to its distinct climate, diverse vegetation, and conservation concerns. This study hypothesizes that protected areas exhibit higher plant diversity and biomass than unprotected areas, primarily due to the impact of protection measures on domestic and wild animal activities. We assessed the abundance and diversity of native vegetation across 25 plots in protected and unprotected areas. Our findings revealed 67 native plant species, including 39 herbs, 18 shrubs, and 10 trees. Key tree species such as Grewia opptiva, Vachellia nilotica, and Senegalia modesta demonstrated higher basal areas in protected areas. In contrast, shrub species like Rhazya stricta, Withania coagulans, and Gymnosporia royleana exhibited higher densities in these areas. Species of conservation concern, such as critically endangered W. coagulans and nearly threatened G. royleana, were more prevalent in protected areas, suggesting the effectiveness of conservation measures. Additionally, locally important and consumable medicinal plant species, including Ajuga bracteosa, Amaranthus viridis, Peganum harmala, Sonchus arvensis, and Zygophyllum indicum revealed substantial abundance, richness, and distribution within protected areas. In conclusion, the study indicates that conservation measures, particularly protected areas, play a crucial role in safeguarding and preserving native plant communities and enhancing species richness.