Soils and forages of Punjab, Pakistan, have mineral concentrations that are marginal for forage plant and animal requirements, and deficiencies of Ca can lead to economic losses. The efficacy of various Ca application methods for improving plant tissue Ca concentrations and forage productivity and nutritional value is not well defined. This field study was conducted in Punjab during 2021 and 2022 with the objective of quantifying the effects of Ca biofortification using seed coating or crop fertilization on forage responses of rhodesgrass (Chloris gayana Kunth.), an important regional forage. Treatments were the factorial combinations of two seed coatings (coated with 2 g CaSO4 kg(-1) seed or non-coated) and nine CaSO4 basal or foliar applications arranged in a randomized complete block design with three replications. The nine basal/foliar application treatments were no CaSO4 (control), basal application of 1 Mg ha(-1) (B1), basal application of 2 Mg ha(-1) (B2), foliar application at 0.5% (F0.5), foliar application at 1% (F1), and B1 + F0.5, B1 + F1, B2 + F0.5, and B2 + F1. Seed coating increased plant height (2%), tillers plant(-1) (13%-16%), tillers m(-2) (15%-17%), leaf area (5%-7%), and dry matter harvested (DMH; 9%-13%) over no coating. Tillers plant(-1) and tillers m(-2) increased with increasing basal CaSO4 application rate. Combining greater basal and foliar rates increased plant height (5.8%-8.3%), leaf area (9.6%-10.3%), and DMH (27.0-27.6). Tissue crude protein (33.0%-40.8%), digestibility (2.3%-2.5%), calcium (88%-92%) and sulfur (60%) concentrations also increased. Calcium biofortification using CaSO4 as a basal and foliar treatment or as seed coating improved rhodesgrass DMH and nutritive value.