The Spiny-tailed Lizard (Uromastyx aegyptia), a vulnerable species native to the desert and semi-desert regions of the Middle East, remains poorly understood, particularly regarding its daily activity patterns in northern Saudi Arabia. This study, conducted in the Ha'il region, aimed to examine these patterns, assess the influence of soil temperature on activity, and identify potential threats to the species. The results revealed that soil temperature significantly affected the lizard's activity patterns. During spring, Spiny-tailed Lizards were more active, spending around 25% of the day engaged in various behaviours, while their activity decreased to less than 20% in summer. In autumn and winter, the lizards did not follow a consistent daily activity, becoming active only when surface temperatures exceeded 35 degrees C. The absence of tracks and sightings in January suggests the species enters a state of complete brumation during this month. While no predation events were recorded via trail cameras, human disturbance from livestock and vehicles was observed in spring and summer. Although the disturbance was minor, reducing this type of human-caused disturbance should be taken into consideration when designing any protection programs. Furthermore, the long-term monitoring of this lizard's daily and seasonal activity patterns is recommended in order to better understand its adaptability to environmental changes, especially those driven by climate fluctuations.