The inherent natural antioxidants found in numerous medicinal plants (MPs) play a crucial role in counteracting the detrimental impacts of oxidative stress. MPs contains polyphenols and flavonoids, which serve as scavengers for free radicals, mitigating oxidative stress and offering potential as alternative treatments for a range of human ailments. The significance of this study lies in its chromatographic spectrum identification, providing a in-depth exploration of the antioxidant capacities and toxicological profiles within the genetic resources of Argyrolobium arabicum. This study described the chromatographic, spectroscopic, antioxidant and hepatoprotective actions of A. arabicum leaves collected from arid zones of Saudi Arabia. Argyrolobium arabicum chromatographic separations of bioactive compounds (HPLC detection isolate prominent compounds D-Pinitol and vitexin) and GC-MS spectra identified five phenolics and six flavonoids organic compounds. FT-IR spectroscopy revealed prominent functional group of alcohols and carboxylic acids bonds stretching. The hepatic toxicity and protective effects were notably administrated of A. arabicum extracts at a dosage of 300 mg/kg, leading to the restoration of ALT levels to 51 (U/L), AST levels to 71.6 (U/L), ALP levels to 169 U/L, and total bilirubin levels to 0.58 (g/dL), which had been altered by the negative control. This research basically contributing to the understanding of medicinally important A. arabicum potential applications in pharmaceutical and environmental fields and used as to elucidate the complex genomic mechanisms explaining the bioactivity and safety of medicinal species.