Edaphoclimatic conditions of agroecosystems, genotypic characteristics of the plant and the management system can modify the nutritional quality of cactus pears (Opuntia ficus-indica Mill). This work evaluated the influence of agroecosystems and production systems on the bromatological composition of cactus pear cladodes. Five agroecosystems were studied: 1 - Irrigated District of Cera & iacute;ma, Guanambi-BA (14 degrees 17' 40" S; 42 degrees 42' 44" W); 2 - Iuiu Valley, Iuiu-BA (14 degrees 23' 50" S; 43 degrees 27' 07" W); 3 - Mania & ccedil;u, Caetit & eacute;-BA (13 degrees 48' 50" S; 42 degrees 24' 32" W); 4 - Baixio, Riacho de Santana-BA (13 degrees 32' 08" S; 43 degrees 09' 19" W); 5 - Morrinhos, Guanambi-BA (14 degrees 14' 02" S; 42 degrees 37' 08" W). Cactus pear tissue samples were collected from August to September 2017. A hierarchical design was used: plant plots were arranged in three repetitions at each cactus pear production system, represented by four farms within five agroecosystems, totaling 20 properties of traditional growers. The bromatological variables most affected by the agroecosystem were organic matter and ash in the cladodes. The production systems adopted by the traditional cactus pear growers affected most of the contents of dry matter, nitrogen, crude protein, and neutral detergent fiber. Plant genotype influenced the composition of neutral detergent fiber of the cladodes to a greater extent.