Quantitative assessments of the impacts of climate change and anthropogenic activities on runoff help us to better understand the mechanisms of hydrological processes. This study analyzed the dynamics of mountainous runoff in the upper reaches of the Shiyang River Basin (USRB) and its sub-catchments, and quantified the impacts of climate change and human activities on runoff using the improved double mass curve (IDMC) method, which comprehensively considers the effects of precipitation and evapotranspiration on runoff, instead of only considering precipitation as before. The results indicated that the annual runoff depth in the USRB showed a slightly increased trend from 1961 to 2018, and sub-catchments were increased in the west and decreased in the east. The seasonal distribution pattern of runoff depth in the USRB and its eight sub-catchments all showed the largest in summer, followed by autumn and spring, and the smallest in winter with an increasing trend. Quantitative assessment results using the IDMC method showed that the runoff change in the USRB is more significantly affected by climate change, however, considerable differences are evident in sub-catchments. This study further developed and improved the method of runoff attribution analysis conducted at watershed scale, and these results will contribute to the ecological protection and sustainable utilization of water resources in the USRB and similar regions.