A comprehensive ecohydrological analysis is designed to understand the formation and evolution of lake Lop Nur and the environmental change over the Tarim River basin. Three temporal scales from century-based climatological mean to decade-based quasi-steady state change and to annual-scale-based abrupt change test are included. Combining the Budyko and Tomer-Schilling framework, this research first analyzes hydroclimatic and ecohydrological resistance/resilience conditions, then attributes observed changes to external/climate impact or to internal/anthropogenic activities, and finally diagnoses the possible tipping point on ecohydrological dynamics. (i) The arid regions reveal less sensitivity in terms of low variabilities of excess water W and energy U and show high resilience, which will more likely stay the same pattern in the future. (ii) Present towns situated in the semiarid regions with a natural hydraulic linkage with the mainstream of the Tarim River show a higher sensitivity and likelihood to be affected if drier scenarios occurred in the future. (iii) The attribution from two subsequent quasi-steady states indicates increasing effects of the anthropogenic activities increase (1961-80 versus 1981-2000) and provides climatical evidence that the central Tarim River basin was getting wetter before 1960 and then kept drying afterward. (iv) In the Kongqi subcatchment, the excess water reveals a significant decrease-then-increase evolution, from which the years with abrupt changes are observed in the 1960s. Generally, both points iii and iv are in agreement with that the closed-basin lake Lop Nur desiccation until the 1970s and its connection with the eastern part of the Taklamakan Sand Sea.