Salinity is considered a vital problem in the vineyard especially which are grown in arid and semi-arid soil which limits the increase in yield of vines. The current study aimed to evaluate the sensitivity of 'Flame seedless' vines to soil salinity which grew on four rootstocks ('Self grafted', 'Freedom', '140 Ruggeri', and '1103 Paullson') during the development stages of berry of seasons (2018-2019). The evaluation has conducted at four during the development stages of berry are flowering (take 4-weeks), fruit set (2 weeks after flowering), Veraison (at the beginning berry color), and at harvesting time. The determination is photosynthetic pigments such as chlorophyll (Chls) and carotene (Car), and chlorophyll fluorescence parameters (F-v/F-m ratio, F-m, and F-0). Also, the carbohydrate of shoots accumulation and the antioxidant enzyme activities (AEAs) have been monitored during the development of the grape berry. The result confirmed that the '1103 Paullson' rootstock presented greater tolerance to soil salinity stress in comparison to other rootstocks in the course of the development stages of the grape berry. Therefore, it is supported by grape growers to graft 'Flame seedless' vine on the of '1103 Paullson' in arid and semi-arid soil. Consequently, it mitigates soil salinity stress in a new 'Flame seedless' vineyards.