Pomegranate (Punica granatumL.) is one of the most important Iranian horticultural products, which is usually cultivated in arid and semi-arid regions. Climate change has led to drought stress and it's a threat to the agricultural products as well as pomegranate. This experiment was conducted on two-year-old rooted pomegranates of eight commercial cultivars namely 'Malase Saveh' ('M-Saveh'), 'Malase Isfahan' ('M-Isfahan'), 'Robabe Ghermeze Shiraz' ('Robab'), 'Gabrie Yazd' ('G-Yazd'), Gabrie Torshe Yazd' ('GT-Yazd'), 'Zaghe Sefide Yazd' ('ZS-Yazd'), 'Zaghe Torshe Yazd' ('ZT-Yazd') and 'Malase Torshe Pishva' ('M-Pishva'). Irrigation treatments were 50% of maximum allowable deficiency (without stress) and 80% of maximum allowable deficiency (stress). This research carried out as a factorial experiment based on randomized complete block design. Increased proline content was variable in pomegranate cultivars exposed to stress, so that, the highest increase was observed in the 'M-Saveh' with a 20.27% increase compared to the control treatment. 'Robab' showed the lowest increase in proline content. The highest increase in superoxide dismutase and peroxidase activity was 8.49 and 16.84%, respectively, in 'ZS-Yazd'. The activity of superoxide dismutase in 'Robab', which was exposed to drought stress, decreased. This cultivar showed the highest electrolyte leakage with an average of 83.58%. The lowest electrolyte leakage was belonged to 'ZS-Yazd' (24.1%), and then 'GT-Yazd' (18.66%). Cluster analysis divided the genotypes into four main groups. 'ZS-Yazd' and 'M-Saveh' were in the same group. These two cultivars were more tolerant to drought stress than other cultivars in the physiological aspects.