center dot Grazing regimes affect the distribution of soil aggregates. center dot Grazing regimes affect the distribution of soil organic carbon (SOC). center dot Differences in SOC concentration and stock were mainly found in the 10-20 cm soil. center dot Moderate grazing is favorable to increase grassland SOC storage. Soil organic carbon (SOC) reflects soil quality and affects ecosystem productivity and the capacity for carbon sequestration. Grazing regimes modulate the status of SOC in grasslands, but their impacts on SOC distribution among soil aggregates are not entirely clear. In this study, the effects of grazing with different intensities and no-grazing with varied enclosure durations were investigated. Increasing grazing intensity enhanced the proportion of 150-53 & micro;m aggregates, while reducing larger aggregates. After 5 years of enclosure, the distribution of soil aggregates in heavily degraded grassland recovered to resemble that in light-grazed grassland. Heavy grazing decreased SOC concentration in the 10-20 cm layer, and SOC concentration in different aggregate sizes decreased with increasing grazing intensity. Differences in SOC stocks among management measures were mainly reflected in the 10-20 cm soil layer, with heavy grazing significantly reducing SOC stocks. SOC stocks were lowest in 2000-250 & micro;m and 250-150 & micro;m aggregates and highest in <20 & micro;m aggregates. Moderate grazing enhanced SOC stocks in <20 & micro;m aggregates. In the 10-20 cm soil layer, 10 years of enclosure significantly decreased SOC stocks in 2000-250 & micro;m, 250-150 & micro;m and <20 & micro;m aggregates compared to 5 years of enclosure. It indicated that grazing regimes affected grassland carbon sequestration and its distribution in soil aggregates, and moderate grazing had a better positive effect on carbon sequestration. This study provides novel insights into the patterns underlying changes in soil organic carbon from soil aggregates perspective.