Advances in research on mineral nutrition interactions among scion-rootstock combinations support the development of more precise fertilization strategies. This study evaluated soil fertility dynamics in a high-density mango orchard with cultivars 'Palmer', 'Tommy Atkins', 'Kent', and 'Keitt' grafted onto the polyembryonic rootstocks Capucho, Coquinho, and Espada. The experiment followed a 4 & times; 3 factorial scheme, with five replications and two plants per plot. Soil samples were collected at 180, 360, and 540 days after transplanting to determine pH, available P and K+, exchangeable Ca2+ and Mg2+, potential acidity (H + Al & sup3;(+)), Cu2+, Fe2+, Mn2+, Zn2+, Na+, and electrical conductivity. Data were subjected to analysis of variance and multivariate analysis. Most soil chemical attributes were not significantly influenced by scion-rootstock combinations over time, indicating that soil fertility during orchard establishment was mainly affected by fertilization management and nutrient uptake. However, significant interactions were observed for exchangeable Ca2+ and Cu2+ at 360 days, as well as for pH and H + Al & sup3;(+) at specific periods, demonstrating that certain scion-rootstock combinations can modulate soil chemical dynamics. Overall, soil pH, Ca2+, and Cu2+ remained within recommended ranges for mango cultivation, while electrical conductivity indicated absence of salinity. Multivariate analysis showed that soil variability was primarily associated with fertility-related nutrients and secondarily with micronutrient availability. These results indicate that rootstock-scion combinations exert limited but measurable effects on soil fertility during orchard establishment in irrigated semi-arid systems under tropical conditions relevant to modern high-density mango production.