Soil salinity is a major threat to crop pro-duction worldwide, particularly in arid regions of the world as global climate change is hastening the pro-cess of soil salinization. The development of cultivars that are salt tolerant, however, offers a more afford-able, sustainable option. Hydroponic systems have evolved into a quick technique for evaluating a large number of genotypes for salt stress at the seedling stage. One hundred forty wheat genotypes including bread wheat, durum and dicoccum wheat with check cultivars were evaluated at three different salinity lev-els along with control under hydroponic conditions. The relevance of seedling traits in screening salt tol-erance was shown by research on correlation, PCA (Principal Component Analysis), and broad sense heritability ( h2bs ). Salinity stress considerably reduced all seedling characteristics under study in terms of growth and physiological variables. All saline treat-ments exhibited high heritability (> 60%) for seedling parameters, including root length, shoot length, fresh weight, and dry weight. PCA and the Stress Tolerance Index (STI) identified the genotypes UASBW-13903, UASBW-13892, DIC-4, DIC-22, DIC-77, DIC-92, DIC-104, and DDK50507 as salt-tolerant, as they exhibited the smallest reductions in seedling traits under saline treatments. Based on the findings, a con-centration of 150 mM NaCl is found suitable and rec-ommended for hydroponic culture-based preliminary salt tolerance screening of various wheat genotypes.