In vitro, screening and selection of drought (low moisture stress) tolerant Vitis species were effectively carried out using polyethylene glycol (PEG) with a molecular weight of 6000 at various concentrations. The inherent strategies implemented by different Vitis species were investigated to better understand of their responses to moisture stress. The findings revealed a notable effect of PEG-induced moisture stress on diverse growth and biochemical attributes across different Vitis genotypes. Analysis of the mortality rates revealed as reflected in lethal dose 50 (LD50) indicated notable differences among the eight Vitis genotypes. Osmolytes, such as proline content and total soluble sugars, were higher in the leaves of tolerant genotypes compared to those that were susceptible. Specifically, the genotype 110R demonstrated the maximum proline levels (2.70 mu M g(-1) FW) and total soluble sugars (25.60 mg g(-1 )FW). In contrast, the highest malondialdehyde levels were recorded in the PN genotype (3.57 mu M g(-1 )FW), followed by MH (3.06 mu M g(-1 )FW). Furthermore, the activity levels of diverse antioxidant enzymes, including catalase, superoxide dismutase, peroxidase, and glutathione reductase increased in plants grown in PEG-supplemented media. This enhancement showed significant variation between susceptible and tolerant genotypes, offering crucial discernment into their adaptive strategies. These results contribute significantly to the knowledge of in vitro screening and selection processes for low moisture stress tolerance. This research offers the potential to foster advancements in development of more resilient Vitis genotypes, somaclones, in vitro-induced mutants, and related studies, potentially leading to improved horticultural practices in arid climates.