Microplastics are persistent contaminants across all environmental matrices. However, there is a paucity of studies conducted in semi-arid aquatic environments. The present study investigated the seasonal variations in the distribution and characteristics of microplastics in water samples of Man Sagar Lake in northwestern India. The findings revealed that the average abundance of microplastics in samples was notably lower during the pre-monsoon season (42.93 +/- 29.72 particles/L) compared to the post-monsoon season (70.54 +/- 36.53 particles/L). A significant difference in microplastic abundance across different sampling locations was observed between the two seasons (F = 7.82, p < 0.0001). The majority of microplastics present in the samples during both seasons measured less than 500 mu m in size, with fragments and fibers being the predominant shapes, and transparent being the most common-colored microplastic. The most abundant polymers detected were polyethylene, polypropylene, and polystyrene. Microplastic contamination factor (MCf) and Pollution Load Index (PLI >1) indicated a moderate to high level of microplastic contamination across all the sampling locations in both seasons. Furthermore, the potential ecological risk index (PERI) indicated that nearly 70 % of the lake area falls under the category of very high ecological risk in both seasons. Additionally, Spearman's correlation and PCA were employed to assess the interactions of microplastics with water quality parameters and potential source identification, respectively. The study's findings could help in developing region-specific remediation and prevention strategies by identifying high-risk areas and potential sources contributing to microplastic contamination.