The high level of boron in dryland and semi-arid soils is an important issue that strongly affects growing and developing crops, especially under drought stress. Meanwhile, brassinosteroid (BR) as a noval stress hormone can improve resistance to abiotic stress in plants. To explore the appropriate foliar application of boron (0.5 and 1 %) and 24-Epi-brassinolide (0.5 and 1 mu M) and their combinations on camelina (Camelina sativa L.) under irrigation regimes a field experiment was conducted during 2018-2020 years. Irrigation regime consisted of well irrigation from emergence until the end of the growing season (WI0), withholding irrigation from flowering to siligue formation (WI1), and withholding irrigation from siligue formation to harvest (WI2)]. Our finding revealed that boron had a destructive effect on phytochemical parameters of camelina while application of brassinolide mitigated the boron impacts and improved the parameters. Results showed the highest increase in chlorophyll a (26.8 and 23.8 %) at B0.5 + BR0.5 treatments under WI1 and WI2 conditions, respectively. Application of BR (0.5 and 1 mu M) and low level of B (B0.5 %) combination alleviated drought stress by improving osmolyte accumulation (proline) (5-9% increase), antioxidant enzymes, superoxide dismutase (SOD) (5.35-5.72 % increase), catalase (CAT) capacity (4.10 % increase) and secondary metabolites (total phenol and flavonoid) (6.78-10.26 % and 4.60-5.27 % increase). Further, malondealdehide (MDA) decreased (8.73 %) at BR and B combination and increased with a high level of B (B1%) application underwithholding irrigation. All of these results confirmed that BR and B synergistically (mainly B0.5 + BR0.5 and B0.5 + BR1) regulate the phytochemical properties response in the camelina plant to drought stress.