Amidst the intensification of global climate change and the increasing impacts of human activities, ecosystem patterns and processes have undergone substantial transformations. The distribution and evolutionary dynamics of mountain ecosystems have become a focal point in ecological research. The Surkhan River Basin is located in the transitional zone between the arid inland regions of Central Asia and the mountain systems, where its unique physical and geographical conditions have shaped distinct patterns of vertical zonation. Utilizing Landsat imagery, this study applies a hierarchical classification approach to derive land cover classifications within the Surkhan River Basin. By integrating the NDVI (normalized difference vegetation index) and DEM (digital elevation model (30 m SRTM)), an "NDVI-DEM-Land Cover" scatterplot is constructed to analyze zonation characteristics from 1980 to 2020. The 2020 results indicate that the elevation boundary between the temperate desert and mountain grassland zones is 1100 m, while the boundary between the alpine cushion vegetation zone and the ice/snow zone is 3770 m. Furthermore, leveraging DEM and LST (land surface temperature) data, a potential energy analysis model is employed to quantify potential energy differentials between adjacent zones, enabling the identification of ecological transition areas. The potential energy analysis further refines the transition zone characteristics, indicating that the transition zone between the temperate desert and mountain grassland zones spans 1078-1139 m with a boundary at 1110 m, while the transition between the alpine cushion vegetation and ice/snow zones spans 3729-3824 m with a boundary at 3768 m. Cross-validation with scatterplot results confirms that the scatterplot analysis effectively delineates stable zonation boundaries with strong spatiotemporal consistency. Moreover, the potential energy analysis offers deeper insights into ecological transition zones, providing refined boundary identification. The integration of these two approaches addresses the dimensional limitations of traditional vertical zonation studies, offering a transferable methodological framework for mountain ecosystem research.