A field experiments was conducted at the Wenxi experimental site of Shanxi Agriculture University, China A study was designed to evaluate the effect of different planting density and tillage conditions, spike, protein content, protein yield and grain yield of dryland wheat cultivar (Liangxing-99). The five doses of nitrogen application included were the following: 150 kg.hm(-2), 225 kg.hm(-2), 300 kg.hm(-2), 375 kg.hm(-2), and 450 kg.hm(-2). The nitrogen rate/amount has extremely significant effects on the number of ears, thousand-grain weight and yield and water use efficiency of winter wheat. Nitrogen absorption and fertilizer productive efficiency significantly were increase by 50 % and 51 %, respectively, while nitrogen harvest index, the number of ears and yield significantly increased by 22 %, 46% and the yield by 25%, 55%. The water use efficiency significantly increased by 33 %. The treatment significantly improved grain albumin 13 %, gliadin 14 %, gluten protein 17 %, total protein 14 %, and grain/alcohol ratio 3 %; wet gluten content increased by 23 %, water absorption by 14 % and, the softening degree was reduced. Improving nitrogen uptake at different growing stages, ultimately increased yield and improved quality. GS activity of flag leaf was closely related with the total grain protein, glutenin, and gliadin content at the middle filling stage. Wide space sowing in Jinnan area was beneficial and increased water consumption during the growth period, also tiller dynamics, promoted nutrient operation, and increased yield and grain protein content.