Previous research has evidenced which skills are required for reading acquisition and which methods are effective for teaching reading. However, recent research indicated that teachers lack sufficient knowledge about the constructs involved in reading instruction. The purpose of this exploratory study was to examine Spanish teachers' practice and opinions on reading instruction. Two samples of Spanish teachers, 840 Preschool teachers and 876 Primary teachers, were surveyed about their opinion on reading skills, their reading instruction practices, and methods of detecting and assessing reading difficulties. The questionnaire for primary teachers also included questions on preparedness and knowledge. Most teachers favored whole-word methods, included maturity and motivation, as relevant aids for phonological awareness and showed poor grasp of factors underpinning reading acquisition. Teachers showed inconsistent and limited knowledge of the evidence-based approach for effective reading instruction. Specific programs are needed to provide preservice teachers with evidence-based instruction and continuous training for in service teachers.