Adoption behavior and physiological stress responses following offspring loss in Pardosa lugubris spider
Abstract
Parental care increases offspring survival and thus contributes to the reproductive success of a species. However, offspring loss may induce behavioral and physiological stress responses in parents. By examining stress markers—heat shock proteins and reactive oxygen species—alongside behavioral observations, we analyzed the stress responses in Pardosa lugubris females following the removal of their egg sacs or juveniles. Stress markers were measured in both females and juveniles. Behavioral trials were conducted to assess maternal responses to egg sac loss: unfertilized females, females adopting foreign egg sacs, and females given a choice between their own and a foreign sac. The results indicate that fertilized females tend to adopt egg sacs after offspring loss, even when the sac is not their own. Offspring removal induced measurable stress responses in both mothers and juveniles, which decreased over time. These findings highlight how offspring loss affects maternal behavior and stress physiology in Pardosa lugubris, offering insight into the mechanisms underlying parental investment and resilience in invertebrates.
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All data generated or analysed during this study are included in this published article and its supplementary information files.
References
Alonso-Alvarez, C. & Velando, A. Benefits and costs of parental care. In The Evolution of Parental Care, 40–45 (Oxford Univ. Press, 2012).
Bleu, J. et al. Reproductive allocation strategies: A long-term study on proximate factors and temporal adjustments in a viviparous lizard. Oecologia 171, 141–151 (2013).
Stearns, S. C. The Evolution of Life Histories (Oxford Univ, 1992).
Costa, J. T. The other insect societies: Overview and new directions. Curr. Opin. Insect. Sci. 28, 40–49 (2018).
Glenszczyk, M. et al. The apple of discord: Can spider cocoons be equipped with antimicrobial factors? - a systematic review. Front. Zool. 22, 9 (2025).
Austin, A. D. The function of spider egg sacs in relation to parasitoids and predators, with special reference to the Australian fauna. J. Nat. Hist. 19, 359–376 (1985).
Foelix, R. F. Biology of Spiders 3rd edn. (Oxford University Press, 2011).
Ewunkem, A. J. & Agee, K. Spider parental care and awe-inspiring egg sac (cocoon). Int. J. Zool. 2022, 6763306 (2022).
Colancecco, M., Rypstra, A. L. & Persons, M. H. Predation and foraging costs of carrying egg sacs of different mass in the wolf spider Pardosa milvina. Behaviour 144, 1003–1018 (2007).
Culley, T., Wiley, J. E. & Persons, M. H. Proximate cues governing egg sac discrimination and recognition in the wolf spider Pardosa milvina (Araneae: Lycosidae). J. Arachnol. 38, 387–390 (2010).
Ruhland, F., Chiara, V. & Trabalon, M. Age and egg-sac loss determine maternal behaviour and locomotor activity of wolf spiders (Araneae, Lycosidae). Behav. Process. 132, 57–65 (2016).
Eason, R. R. Life history and behavior of Pardosa lapidicina Emerton (Araneae: Lycosidae). J. Kans. Entomol. Soc. 42, 339–360 (1969).
Preston-Mafham, K. & Preston-Mafham, R. The Natural History of Spiders. (Crowood Press, 1996).
Ruhland, F., Pétillon, J. & Trabalon, M. Physiological costs during the first maternal care in the wolf spider Pardosa saltans (Araneae, Lycosidae). J. Insect Physiol. 95, 42–50 (2016).
Trabalon, M. et al. Embryonic and post-embryonic development inside wolf spiders’ egg sac with special emphasis on the vitellus. J. Comp. Physiol. B 188, 211–224 (2017).
Berry, A. D. & Rypstra, A. L. Egg sac recognition and fostering in the wolf spider Pardosa milvina (Araneae: Lycosidae) and its effects on spiderling survival. Behav. Ecol. Sociobiol. 75, 1–9 (2021).
Ewunkem, J. A., Ntonifor, N. N. & Parr, M. C. Bioecology of Heteropoda venatoria (L.) (Araneae: Sparassidae) and its implications in a tropical banana agroecosystem. J. Glob. Agric. Ecol. 5, 164–175 (2016).
Circu, M. L. & Aw, T. Y. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic. Biol. Med. 48, 749–762 (2010).
Nordberg, J. & Arnér, E. S. J. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic. Biol. Med. 31, 1287–1312 (2001).
Pandey, B. N. & Mishra, K. P. In-vitro studies on radiation-induced membrane oxidative damage in apoptotic death of mouse thymocytes. Int. J. Low Radiat. 1, 113–119 (2003).
Carpenter, C. M. & Hofmann, G. E. Expression of 70kDa heat shock proteins in Antarctic and New Zealand notothenioid fish. Comp. Biochem. Physiol. A 125, 229–238 (2000).
Tkáčková, M. & Angelovičová, L. Heat-Shock Proteins (HSPs): A review. Sci. Papers: Anim. Sci. Biotechnol. 45, 349 (2012).
Adamo, S. A. The effects of the stress response on immune function in invertebrates: An evolutionary perspective on an ancient connection. Horm. Behav. 62, 324–330 (2012).
Chaitanya, R. K., Shashank, K. & Sridevi, P. Oxidative stress in invertebrate systems. Free Radicals Dis. 19, 51–68 (2016).
Wilczek, G. Apoptosis and biochemical biomarkers of stress in spiders from industrially polluted areas exposed to high temperature and dimethoate. Comp. Biochem. Physiol. C 141, 194–206 (2005).
Dolejš, P., Kubcová, L. & Buchar, J. Reproduction of Arctosa alpigena lamperti (Araneae: Lycosidae) – where, when, how, and how long?. Invertebr. Reprod. Dev. 56, 72–78 (2012).
Salmon, A. B., Marx, D. B. & Harshman, L. G. A cost of reproduction in Drosophila melanogaster: Stress susceptibility. Evolution 55, 1600–1608 (2001).
Sørensen, J. G., Kristensen, T. N. & Loeschcke, V. The evolutionary and ecological role of heat shock proteins. Ecol. Lett. 6, 1025–1037 (2003).
Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970).
Costantini, D. Oxidative stress and hormesis in evolutionary ecology and physiology. Marriage Between Mech. Evol. Approach. https://doi.org/10.1007/978-3-642-54663-1 (2014).
Feder, M. E. & Hofmann, G. E. Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology. Annu. Rev. Physiol. 61, 243–282 (1999).
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M.S. conceived and designed the study, prepared the study material, and wrote the main manuscript text. M.S., A.Cz., M.G., W.P., K.C-Ś., and A.B. performed the experiments, analyzed, and interpreted the data. M.S. and B.Ł. conducted the statistical analysis. A.Cz., M.G., and B.Ł. contributed to the review and editing of the manuscript.
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Sawadro, M., Czerwonka, A., Łozowski, B. et al. Adoption behavior and physiological stress responses following offspring loss in Pardosa lugubris spider. Sci Rep (2025). https://doi.org/10.1038/s41598-025-30418-2
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DOI: https://doi.org/10.1038/s41598-025-30418-2