Single-atom catalysts (SACs) with high metal use and catalytic activity have been widely used in energy and biomedicine. However, the mass production of stable and homogeneous SACs still faces major challenges. Here we report a generalizable strategy for producing highly stable, low-cost SACs in a scalable and sustainable way. After screening 19,196 possible atom-active structures on the surface of NiTi substrate by combining quantum chemistry with machine learning, we synthesized 22 SACs in an area coverage up to 200 cm² per preparation within 1 hour. The bimetallic CoV and PtCu SACs exhibit the highest biocatalytic activity, which is superior to that of natural enzymes and remains stable after 4 years. Moreover, these single-atom embedded NiTi vascular stents allow for the removal of excessive nitrides and oxides by continuous multi-enzyme catalysis, protecting them from vascular injury, with the corresponding vessel size being three times larger than that of the bare NiTi stent after 3 months of implantation, close to healthy vessels. This single-atom manufacturing strategy could be extended to other biomedical devices with high sustainability and bioactivity.